Alexander L. Chernorudskiy：PhD, Professor, Principal Investigator
Education
2010-2012: Post-doctoral training in molecular medicine; Structured International Post Doctoral Program (SIPOD), European School of Molecular Medicine (SEMM), Milan, Italy
2004-2009: PhD in biochemistry and physiology; Department of Human and Animal Physiology and Biochemistry, Nizhny Novgorod State University, Nizhny Novgorod, Russia
PhD thesis title: Regulation of proteins functional properties by site-specific ubiquitylation
1999-2004: MSc in biology; Department of Human and Animal Physiology and Biochemistry, Nizhny Novgorod State University, Nizhny Novgorod, Russia
MSc thesis title: Complex approach to the isolation and identification of intracellular ubiquitin-protein conjugates
Research Experience
2015-2024: Senior researcher; Signal Transduction Laboratory, Mario Negri Institute for Pharmacological Research, Milan, Italy
Research project: Identification and functional characterization of redox-sensitive molecular targets associated with neuromuscular diseases and cancer (supervisor – Dr. Ester Zito).
2012-2014: Research associate, project leader; Postgenomic Technology Group, Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia
Research project: Studying the mechanisms of ubiquitin signal recognition (PI role with independent funding).
2010-2012: Post-doctoral fellow; Endocytosis, Signalling and Cancer Laboratory, IFOM –FIRC Institute of Molecular Oncology, Milan, Italy
Research project: Studying the specificity of ubiquitin recognition and signalling (supervisor – Prof. Pier Paolo Di Fiore).
2009-2010: Acting group leader (substituting Dr. Gainullin during his sabbatical leave); Postgenomic Technology Group, Institute of Applied and Fundamental Medicine, Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia
2006-2009: Research assistant; Postgenomic Technology Group, Institute of Applied and Fundamental Medicine, Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia
Research project: Proteomics and bioinformatics approach to the study of protein ubiquitylation (supervisor – Dr. Murat Gainullin).
2006-2008 (part-time): Research intern; Membrane Dynamics Laboratory, Dept. of Intracellular Signalling and Transport, Institute of Cytology RAS, St. Petersburg, Russia
Research project: Role of ubiquitylation in regulation of EGFR endocytosis and intracellular sorting (supervisor – Prof. Elena Kornilova).
2005: Summer research student; DNA Damage Tolerance Laboratory, Cancer Research UK London Research Institute, UK
Research project: Mechanistic analysis of a ubiquitin protein ligase in Saccharomyces cerevisiae (supervisor – Dr. Helle Ulrich).
Other professional activities
2013-2015: Freelance writer (medicine and biotechnology); Scientific consultant / editor,
EOL Labs Ltd, Novosibirsk, Russia
Development of UbiProt Database http://ubiprot.org.ru
Project “UbIdent” – development of software tools for proteomic experiments http://ubiprot.org.ru/index.php?mode=ubident
Ad hoc reviewer for Bioinformatics journal (since 2008)
Membership in professional associations
Biochemical Society of the Russian Academy of Sciences
Bioinformatics Organization
Main publications
1. Germani S., Van Ho A.T., Cherubini A., Varone E., Chernorudskiy A., Renna G.M., Fumagalli S., Gobbi M., Lucchetti J., Bolis M., Guarrera L., Craparotta I., Rastelli G., Piccoli G., de Napoli C., Nogara L., Poggio E., Brini M., Cattaneo A., Bachi A., Simmen T., Calì T., Quijano-Roy S., Boncompagni S., Blaauw B., Ferreiro A., Zito E. SEPN1-related myopathy depends on the oxidoreductase ERO1A and is druggable with the chemical chaperone TUDCA. 2024. Cell Rep Med. 5(3): 101439. DOI: 10.1016/j.xcrm.2024.101439
2. Varone E.*, Chernorudskiy A.*, Cherubini A., Cattaneo A., Bachi A., Fumagalli S., Erol G., Gobbi M., Lenardo M.J., Borgese N., Zito E. ERO1 alpha deficiency impairs angiogenesis by increasing N-glycosylation of a proangiogenic VEGFA. 2022. Redox Biol. 56: 102455. DOI: 10.1016/j.redox.2022.102455
* equally contributed authors
3. Varone E., Decio A., Chernorudskiy A., Minoli L., Brunelli L., Ioli F., Piotti A., Pastorelli R., Fratelli M., Gobbi M., Giavazzi R., Zito E. The ER stress response mediator ERO1 triggers cancer metastasis by favoring the angiogenic switch in hypoxic conditions. 2021. Oncogene. 40 (9): 1721-1736. DOI: 10.1038/s41388-021-01659-y
4. Filipe A.*, Chernorudskiy A.*, Arbogast S., Varone E., Villar-Quiles R.N., Pozzer D., Moulin M., Fumagalli S., Cabet E., Dudhal S., De Simoni M.G., Denis R., Vadrot N., Dill C., Giovarelli M., Szweda L., De Palma C., Pinton P., Giorgi C., Viscomi C., Clementi E., Missiroli S., Boncompagni S., Zito E., Ferreiro A. Defective endoplasmic reticulum-mitochondria contacts and bioenergetics in SEPN1-related myopathy. 2021. Cell Death Differ. 28 (1): 123-138. DOI: 10.1038/s41418-020-0587-z
* equally contributed authors
5. Chernorudskiy A., Varone E., Colombo S.F., Fumagalli S., Cagnotto A., Cattaneo A., Briens M., Baltzinger M., Kuhn L., Bachi A., Berardi A., Salmona M., Musco G., Borgese N., Lescure A., Zito E. Selenoprotein N is an endoplasmic reticulum calcium sensor that links luminal calcium levels to a redox activity. 2020. Proc. Natl. Acad. Sci. USA. 117 (35): 21288-21298. DOI: 10.1073/pnas.2003847117
6. Varone E., Pozzer D., Di Modica S., Chernorudskiy A., Nogara L., Baraldo M., Cinquanta M., Fumagalli S., Villar-Quiles R.N., De Simoni M.G., Blaauw B., Ferreiro A., Zito E. SELENON (SEPN1) protects skeletal muscle from saturated fatty acid-induced ER stress and insulin resistance. 2019. Redox Biol. 24: 101176. DOI: 10.1016/j.redox.2019.101176
7. Pozzer D., Varone E., Chernorudskiy A., Schiarea S., Missiroli S., Giorgi C., Pinton P., Canato M., Germinario E., Nogara L., Blaauw B., Zito E. A maladaptive ER stress response triggers dysfunction in highly active muscles of mice with SELENON loss. 2019. Redox Biol. 20: 354-366. DOI: 10.1016/j.redox.2018.10.017
8. Chernorudskiy A.L., Zito E. Regulation of calcium homeostasis by ER redox: a close-up of the ER/mitochondria connection. 2017. J. Mol. Biol. 429 (5): 620-632. DOI: 10.1016/j.jmb.2017.01.017
9. Chernorudskiy A.L., Gainullin M.R. Ubiquitin system: Direct effects join the signaling. 2013. Sci. Signal. 6 (280): pe22. DOI: 10.1126/scisignal.2004251
10. Prakhov N.D., Chernorudskiy A.L., Gainullin M.R. VSDocker: a tool for parallel high-throughput virtual screening using AutoDock on Windows-based computer clusters. 2010. Bioinformatics. 26 (10): 1374-1375. DOI: 10.1093/bioinformatics/btq149
11. Kondratov K.A., Chernorudsky A.L., Amosova A.P., Kornilova E.S. Termination of tyrphostin AG1478 application results in different recovery of EGF receptor tyrosine residues 1045 and 1173 phosphorylation in A431 cells. 2009. Cell Biol Int. 34 (1): 81-87. DOI: 10.1042/CBI20090159
12. Chernorudskiy A.L., Garcia A., Eremin E.V., Shorina A.S., Kondratieva E.V., Gainullin M.R. UbiProt: a database of ubiquitylated proteins. 2007. BMC Bioinformatics. 8 (1): 126. DOI: 10.1186/1471-2105-8-126
13. Chernorudskiy A.L., Shorina A.S., Garcia A., Gainullin M.R. Evaluation of direct effects of protein ubiquitylation using computational analysis. 2006. Biophysics (Oxf.). 51 (S1): P. 39-43. DOI: 10.1134/S0006350906070086
